![]() (2) Gel electrophoresis is used to separate proteins based on their molecular weight. A molecular weight (MW) marker, which contains prelabeled proteins of varied, known molecular weights, is loaded on the gel alongside the protein sample as a size reference. (1) The sample, typically a mixture of proteins, is loaded on the gel. Western blotting is a standard laboratory method that uses antibodies to detect target proteins in a sample. Additional resources include a toolbox to help scientists produce more reproducible western blot data, teaching slides in English and Spanish, and an antibody reporting template. We present detailed descriptions and visual examples to help scientists, peer reviewers, and editors to publish more informative western blot figures and methods. Important antibody identifiers like company or supplier, catalog number, or RRID were omitted frequently for primary antibodies and regularly for secondary antibodies. Western blot methods sections often lack information on the amount of protein loaded on the gel, blocking steps, and antibody labeling protocol. Publishing blots with visible molecular weight markers is rare, and many blots additionally lack molecular weight labels. Our data show that most published western blots are cropped and blot source data are not made available to readers in the supplement. We systematically examined 551 articles published in the top 25% of journals in neurosciences ( n = 151) and cell biology ( n = 400) that contained western blot images, focusing on practices that may omit important information. While several groups have studied the prevalence of image manipulation or provided recommendations for improving western blotting, data on the prevalence of common publication practices are scarce. Unfortunately, poor western blot image display practices and a lack of detailed methods reporting can limit a reader’s ability to evaluate or reproduce western blot results. Western blotting is a standard laboratory method used to detect proteins and assess their expression levels. Weissgerber, Conceptualization, Funding acquisition, Methodology, Project administration, Resources, Supervision, Visualization, Writing – review & editing Lea Scherschinski, Conceptualization, Investigation, Methodology, Validation, Writing – original draft, Writing – review & editing,įernando Raúl Urrutia Gonzalez, Investigation, Validation, Writing – review & editing,Īnd Tracey L. Nico Riedel, Conceptualization, Investigation, Resources, Software, Writing – review & editing, Vartan Kazezian, Conceptualization, Investigation, Methodology, Validation, Writing – review & editing,Īlina Koppold, Investigation, Validation, Visualization, Writing – review & editing,Ĭesar Alberto Moreira Restrepo, Data curation, Investigation, Validation, Writing – review & editing, Max Hellrigel-Holderbaum, Investigation, Methodology, Writing – review & editing, Marinus Fislage, Investigation, Writing – original draft, Writing – review & editing,īiswajit Ghosh, Conceptualization, Investigation, Methodology, Validation, Writing – review & editing, Jeehye An, Data curation, Formal analysis, Project administration, Visualization, Writing – review & editing,Īyça Akan, Data curation, Investigation, Methodology, Project administration, Validation, Writing – review & editing,Įlkhansa Ahmed Mohamed Ali, Investigation, Writing – review & editing,įelix Busch, Conceptualization, Investigation, Methodology, Writing – review & editing, Lydia Jones, Conceptualization, Investigation, Methodology, Writing – original draft, Writing – review & editing, Larissa Breuer, Conceptualization, Investigation, Methodology, Project administration, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing, Cristina Kroon, Conceptualization, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing,
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